Cross-linked Leucaena Seed Gum Matrix: An Affinity Chromatography Tool for Galactose-specific Lectins
نویسندگان
چکیده
منابع مشابه
Affinity Chromatography of Lectins
Affinity chromatography is a technique used to purify compounds, such as proteins, that have the ability to non-covalently and reversibly bind specific molecules, known as ligands. This method differs from the classical chromatography techniques in that the protein is purified on the basis of a unique biochemical property. In affinity chromatography, the ligand is covalently attached to a matri...
متن کاملStudies on Leucaena Leucocephala Seed Gum: Rheological Properties
The apparent viscosity (pseudoplastic viscosity) of mucilage of leucaena seed gum was calculated, based on flow curves, obtamed With multiple point viscometer. Attempts were made to correlate the various rheological properties, describIng the_ overall flow behaviOur of gum, with their concentrations. The relationship between N(exponential constant) and concentration was found to be a zero order...
متن کاملAffinity-repulsion chromatography. Principle and application to lectins.
The interactions of proteins with their immobilized ligands in an electrically charged microenvironment were studied. The binding of lectins to erythrocytes and to affinity matrices was used as a model system. Lectins bind and agglutinate erythrocytes in the presence of at least 10 mM NaCl or 1 mM CaCl2, but not in deionized water. The salt dependence of the agglutination process is due to the ...
متن کاملStudies on disintegrant action of Leucaena leucocephala seed gum in ibuprofen tablet and its mechanism
Leucaena (Leucaena leucocephala) seed gum (LSG) was evaluated for disintegrant action in lactose (soluble) based tablets containing ibuprofen, a relatively insoluble drug. The properties of tablets evaluated include weight uniformity, hardness, friability, disintegration time and in vitro dissolution (k, T 50 , A 30 ). LSG, at low concentration level (2% w/w), can be used as a disintegrant in t...
متن کاملMagnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification.
The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe+2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMB Reports
سال: 2005
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2005.38.3.370